1OFH
Asymmetric complex between HslV and I-domain deleted HslU (H. influenzae)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-15 |
Detector | ADSC CCD |
Spacegroup name | P 6 |
Unit cell lengths | 192.220, 192.220, 115.890 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.500 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g3i |
RMSD bond length | 0.007 |
RMSD bond angle | 1.270 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.073 | 0.319 * |
Total number of observations | 344637 * | |
Number of reflections | 81671 | |
<I/σ(I)> | 39.8 | 2.3 |
Completeness [%] | 97.0 | 74.9 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7 * | PEG-MME 2000, KCL,MG(OAC)2,CITRATE, PH 5.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 15-20 (mg/ml) | |
2 | 1 | 1 | MOPS | 10 (mM) | pH7.0 |
3 | 1 | 1 | magnesium acetate | 1 (mM) | |
4 | 1 | 1 | ATP | 1 (mM) | |
5 | 1 | 2 | PEG2000MME | 7 (%) | |
6 | 1 | 2 | 1 (M) | ||
7 | 1 | 2 | magnesium acetate | 10 (mM) | |
8 | 1 | 2 | citrate | 50 (mM) | pH5.5 |