1OEN

PHOSPHOENOLPYRUVATE CARBOXYKINASE

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1991-06-03
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 21 21 21
Unit cell lengths	77.696, 89.660, 93.836
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.202
Rwork	0.202
R-free	0.24400
RMSD bond length	0.006
RMSD bond angle	24.610 *
Data reduction software	MADNES
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.093 *
Total number of observations	249762 *
Number of reflections	47596 *
Completeness [%]	90.8 *
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop	EDTA	0.1 (mM)
4	1	reservoir	sodium acetate	100 (mM)
5	1	reservoir	PEG8000	15-20 (%)
6	1	reservoir	sodium azide	0.02 (%(w/v))