1OEE

YodA from Escherichia coli crystallised with cadmium ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-10-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	106.470, 47.985, 42.493
Unit cell angles	90.00, 92.77, 90.00

Refinement procedure

Resolution	53.450 - 2.100
R-factor	0.1709
Rwork	0.169
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ZN CRYSTAL FORM OF YODA
RMSD bond length	0.015 *
RMSD bond angle	1.325 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.060	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.072	0.308
Number of reflections	12317
<I/σ(I)>	14.35	3.2
Completeness [%]	93.8	64.9
Redundancy	3.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	6.5		David, G., (2002) Acta Cryst., D58, 1243. *