1OEE
YodA from Escherichia coli crystallised with cadmium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.470, 47.985, 42.493 |
| Unit cell angles | 90.00, 92.77, 90.00 |
Refinement procedure
| Resolution | 53.450 - 2.100 |
| R-factor | 0.1709 |
| Rwork | 0.169 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ZN CRYSTAL FORM OF YODA |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 1.325 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.060 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.072 | 0.308 |
| Number of reflections | 12317 | |
| <I/σ(I)> | 14.35 | 3.2 |
| Completeness [%] | 93.8 | 64.9 |
| Redundancy | 3.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 6.5 | David, G., (2002) Acta Cryst., D58, 1243. * |
