1OE9
Crystal structure of Myosin V motor with essential light chain-nucleotide-free
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Collection date | 2002-11-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.933, 98.247, 111.378 |
| Unit cell angles | 90.00, 101.43, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 * |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mys |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.296 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.120 |
| High resolution limit [Å] | 2.000 * | 2.050 |
| Rmerge | 0.060 | 0.335 |
| Number of reflections | 69115 | |
| <I/σ(I)> | 14 | 2.34 |
| Completeness [%] | 96.8 | 88.4 |
| Redundancy | 2.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 6% PEG8000 (W/V), 50MM MOPS PH 6.5, 2MM DTT, 2MM NAN3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 6 (%) | |
| 2 | 1 | reservoir | MOPS | 50 (mM) | pH6.5 |
| 3 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | 2 (mM) | ||
| 5 | 1 | drop | protein | 8 (mg/ml) |
