1ODZ
Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 131.855, 131.855, 54.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| R-factor | 0.152 |
| Rwork | 0.151 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j9y |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.036 | 0.384 |
| Number of reflections | 171242 | |
| <I/σ(I)> | 29.5 | 2.21 |
| Completeness [%] | 93.7 | 64.4 |
| Redundancy | 4.01 | 2.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100MM TRIS 7.5, 26% PEG550, 9MM ZNSO4, 10MM MANNOTETRAOSE, pH 7.50 |
