1OCY

Structure of the receptor-binding domain of the bacteriophage T4 short tail fibre

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-13
Detector	ADSC CCD
Spacegroup name	P 3 2 1
Unit cell lengths	50.782, 50.782, 435.647
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.500
Rwork	0.143
R-free	0.15200 *
Structure solution method	SIRAS
RMSD bond length	0.018 *
RMSD bond angle	1.600 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE/RESOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.106	0.284 *
Number of reflections	96264
<I/σ(I)>	4.122	2.37
Completeness [%]	89.8	53.3
Redundancy	6.6 *	3.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6		15-25 % (V/V),2-METHYLPROPANE-2-OL (TERTIARY BUTANOL), 100 MM SODIUM CITRATE PH 5.6, 10% (V/V) GLYCEROL,10 MM HEPES, 150 MM SODIUM CHLORIDE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	tertiary butanol	15-35 (%(v/v))
3	1	reservoir	sodium citrate	100 (mM)	pH5.6
4	1	reservoir	glycerol	0-10 (%(v/v))