1OCU
Crystal structure of the yeast PX-domain protein Grd19p (sorting nexin 3) complexed to phosphatidylinosytol-3-phosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Collection date | 2002-07-15 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.430, 55.760, 64.750 |
| Unit cell angles | 110.75, 97.35, 99.49 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ocs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.080 * | 2.330 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.047 | 0.256 * |
| Number of reflections | 11970 * | |
| <I/σ(I)> | 25.1 | 3.2 |
| Completeness [%] | 96.0 | 77.31 * |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 293 * | 10% PEG8000, 8% ETHYLENE GLYCOL, 0.1M NA-HEPES PH 7,5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | diC4PtdIns(3)P | 2 (mM) | |
| 3 | 1 | drop | 0.1 (M) | ||
| 4 | 1 | reservoir | beta-mercaptoethanol | 10 (mM) | |
| 5 | 1 | reservoir | Tris-HCl | 20 (mM) | pH7 |
| 6 | 1 | reservoir | PEG8000 | 10 (%) | |
| 7 | 1 | reservoir | ethlene glycol | 8 (%) | |
| 8 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
