1OCC
STRUCTURE OF BOVINE HEART CYTOCHROME C OXIDASE AT THE FULLY OXIDIZED STATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 189.100, 210.500, 178.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.25200 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.296 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.780 | 0.290 |
| Number of reflections | 155505 | |
| <I/σ(I)> | 19.5 | 2.2 |
| Completeness [%] | 88.8 | 69.6 |
| Redundancy | 3.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.8 | 4 * | pH 6.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | decyl maltoside | 0.2 (%) | |
| 2 | 1 | 1 | sodium phosphate | 40 (mM) | |
| 3 | 1 | 1 | protein | 9.0 (%(w/v)) | |
| 4 | 1 | 1 | PEG4000 |
