1OBU

Apocrustacyanin C1 crystals grown in space and earth using vapour diffusion geometry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	293
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	42.018, 81.033, 110.507
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.940 - 2.000
R-factor	0.177
Rwork	0.174
R-free	0.22700
Structure solution method	OTHER
RMSD bond length	0.034 *
RMSD bond angle	2.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	65.940
High resolution limit [Å]	2.000
Number of reflections	26054
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *		Snell, E.H., (1997) Acta Crystallogr.,Sect.D, 53, 231. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	drop	EDTA	1 (mM)	pH7.0
4	1	reservoir	MPD	5 (%(v/v))
5	1	reservoir	EDTA	1 (mM)
6	1	reservoir	Tris-HCl	0.1 (M)	pH9.0
7	1	reservoir	ammonium sulfate	1.9 (M)