1OB9

Holliday Junction Resolving Enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-09-16
Detector	RIGAKU IMAGE PLATE
Spacegroup name	I 41
Unit cell lengths	76.570, 76.570, 88.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.630 - 2.000
Rwork	0.200
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ob8
RMSD bond length	0.012
RMSD bond angle	1.313
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	50200
<I/σ(I)>	11.9	2.46
Completeness [%]	97.8	95.9
Redundancy	2.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	293	0.1M HEPES PH 7.5,2.0M AMMONIUM FORMATE,PASSED THROUGH MOTHER LIQUOR WITH 20%ETHYLENE GLYCOL,293K