1OAX
Fv Structure of the IgE SPE-7 in complex with acenaphthenequinone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.726, 78.636, 167.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.530 - 2.670 |
| Rwork | 0.238 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ANQ |
| RMSD bond length | 0.207 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 2.980 |
| High resolution limit [Å] | 2.660 | 2.670 |
| Rmerge | 0.234 | |
| Number of reflections | 30262 | |
| <I/σ(I)> | 9.3 | 3.6 |
| Completeness [%] | 97.4 | 91.7 |
| Redundancy | 9.9 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 21% PEG 8K 0.1M NA CACODYLATE, 0.2M NA ACETATE PH5.5, pH 5.50 |
