1OAT

ORNITHINE AMINOTRANSFERASE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	293
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1993-10
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	116.300, 116.300, 190.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.000 - 2.500
R-factor	0.171 *
Rwork	0.185
R-free	0.24300 *
Structure solution method	MOLECULAR REPLACEMENT AND NON-CRYSTALLOGRAPHIC AVERAGING
Starting model (for MR)	DIALKYLGLYCINE DECARBOXYLASE PDB ENTRY 1DGD
RMSD bond length	0.010 *
RMSD bond angle	1.580 *
Data reduction software	MARXDS
Data scaling software	CCP4
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000 *
High resolution limit [Å]	2.500
Rmerge	0.082	0.349 *
Number of reflections	52220
Completeness [%]	99.9	99.9
Redundancy	5.4	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8		pH 7.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	PLP	0.01 (M)
4	1	reservoir	PEG6000	10-15 (%(w/v))
5	1	reservoir		50-200 (mM)