1OAP
Mad structure of the periplasmique domain of the Escherichia coli PAL protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2001-12-15 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9393,0.97953,0.97921 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 88.575, 88.575, 68.028 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.980 - 1.930 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.23400 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.055 | 0.218 |
| Number of reflections | 9422 | |
| <I/σ(I)> | 7.7 | 3.4 |
| Completeness [%] | 96.3 | 97.5 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | MPD 40%, PEG 4000 10%, AMSO4 0.2M, IMIDAZOLE 10MM, MES BUFF, pH 6.00 |
