1OAJ
Active site copper and zinc ions modulate the quaternary structure of prokaryotic Cu,Zn superoxide dismutase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-28 |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 85.900, 85.900, 98.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.730 |
R-factor | 0.191 |
Rwork | 0.183 |
R-free | 0.23100 |
Structure solution method | OTHER |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.037 | 0.178 |
Number of reflections | 14997 | |
<I/σ(I)> | 20 | 5 |
Completeness [%] | 97.5 | 75.8 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 301 | PEG 8,000 25%, NACL 100 MM, SODIUM ACETATE 50 MM, PH 4, TEMPERATURE 28C |