1OAI

Complex between Tap UBA domain and FxFG nucleoporin peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-15
Detector	ASCD
Spacegroup name	P 31 2 1
Unit cell lengths	42.974, 42.974, 66.852
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.000
Rwork	0.149
R-free	0.15800 *
Structure solution method	MIRAS
RMSD bond length	0.023 *
RMSD bond angle	1.940 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	1.050
High resolution limit [Å]	1.000	1.000
Rmerge	0.065	0.357 *
Total number of observations	217401 *
Number of reflections	39136 *
<I/σ(I)>	5.334	1.92
Completeness [%]	100.0	100
Redundancy	5.6 *	4.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	18 *	AS DESCRIBED IN MANUSCRIPT, pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium acetate	100 (mM)	pH6.5
2	1	reservoir	ammonium sulfate	1.9 (M)
3	1	reservoir	dithiothreitol	10 (mM)
4	1	reservoir	EDTA	1 (mM)
5	1	drop	Tap UBA	1.3 (mM)
6	1	drop	FxFG peptide	2.3 (mM)