1OAE
Crystal structure of the reduced form of cytochrome c" from Methylophilus methylotrophus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.688, 74.362, 77.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.950 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | OXIDIZED STRUCTURE OF CYTOCHROME C" |
| RMSD bond length | 0.022 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.860 | 2.050 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.083 | 0.480 |
| Number of reflections | 25348 | |
| <I/σ(I)> | 7.2 | 2.1 |
| Completeness [%] | 97.9 | 87.8 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.00 |
