1OAD
Glucose isomerase from Streptomyces rubiginosus in P21212 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.450, 129.590, 78.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 * - 1.500 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| R-free | 0.18600 |
| Structure solution method | OTHER |
| RMSD bond length | 0.026 * |
| RMSD bond angle | 0.038 * |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 0.150 |
| Number of reflections | 300270 | |
| <I/σ(I)> | 18.8 | 5.6 |
| Completeness [%] | 96.8 | 93.2 |
| Redundancy | 3.5 * | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 17 MG/ML PROTEIN, 12 % MPD, 0.1 M MGCL2, 50 MM TRIS BUFFER PH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH7.0 |
| 3 | 1 | reservoir | MPD | 25 (%) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | Tris | 0.1 (M) | pH7.0 |
