1OA8
AXH domain of human spinocerebellar ataxin-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-15 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.960,0.97916,0.97966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.783, 82.441, 137.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.700 * - 1.700 |
| Rwork | 0.210 |
| R-free | 0.25200 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.780 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.700 * | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.065 | 0.416 |
| Total number of observations | 267655 * | |
| Number of reflections | 62044 | |
| <I/σ(I)> | 5.9 | 1.7 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 4.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | PROTEIN WAS CRYSTALLIZED FROM 0.1M TRIS/HCL, PH 7.0, 0.2M AMMONIUM SULPHATE, 5 MM DTT, 15% PEG 4000, 20% GLYCEROL, AT 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.8 |
| 2 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
| 3 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 16 (%) | |
| 5 | 1 | reservoir | glycerol | 20 (%) | |
| 6 | 1 | drop | protein | 20 (mg/ml) | |
| 7 | 1 | drop | Tris | 10 (mM) | pH7.0 |
| 8 | 1 | drop | dithiothreitol | 5 (mM) |
