1O9Y
Crystal structure of the C-terminal domain of the HrcQb protein from Pseudomonas syringae pv. phaseolicola
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9800,0.9804,0.8856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.018, 27.696, 98.850 |
| Unit cell angles | 90.00, 99.68, 90.00 |
Refinement procedure
| Resolution | 39.000 - 2.300 * |
| R-factor | 0.18468 |
| Rwork | 0.182 |
| R-free | 0.22800 * |
| Structure solution method | MAD |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.261 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.420 |
| High resolution limit [Å] | 2.300 * | 2.300 * |
| Rmerge | 0.156 * | |
| Number of reflections | 12352 * | |
| <I/σ(I)> | 9.3 | 4.6 |
| Completeness [%] | 0.1 * | 88.6 |
| Redundancy | 3.4 * | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 292 * | Fadouloglou, V.E., (2001) Acta Cryst., D57, 1689. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MPD | 30 (%) | |
| 2 | 1 | drop | sodium cacodylate | 100 (mM) | pH6.5 |
| 3 | 1 | drop | magnesium acetate | 200 (mM) | |
| 4 | 1 | reservoir | MPD | 13-15 (%(v/v)) | |
| 5 | 1 | reservoir | magnesium acetate | 70-90 (mM) | |
| 6 | 1 | reservoir | bis-tris | 100 (mM) | pH6.4-6.6 |
