1O9T
Methionine adenosyltransferase complexed with both substrates ATP and methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 114.990, 114.990, 160.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.700* |
| R-factor | 0.265 |
| Rwork | 0.265 |
| R-free | 0.29000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qm4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.600 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.920 |
| High resolution limit [Å] | 2.700 * | 2.700 * |
| Rmerge | 0.078 * | 0.502 * |
| Number of reflections | 27790 * | |
| <I/σ(I)> | 7.7 | 1.7 |
| Completeness [%] | 97.5 | 97.5 * |
| Redundancy | 4.1 | 3.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 16% PEG 10000, 10MM L-METHIONINE, 100MM HEPES PH=7.5 (SOAKING IN 30 MM ATP SOLUTION), pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH8. |
| 3 | 1 | drop | dithiothreitol | 10 (mM) | |
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | drop | EDTA | 1 (mM) | |
| 7 | 1 | reservoir | PEG10000 | 16 (%(w/v)) | |
| 8 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
