1O9H

rRNA methyltransferase aviRa from Streptomyces viridochromogenes at 2.4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200B
Temperature [K]	287
Detector technology	AREA DETECTOR
Collection date	2002-01-11
Detector	BRUKER
Spacegroup name	P 1 21 1
Unit cell lengths	37.098, 48.937, 63.714
Unit cell angles	90.00, 99.05, 90.00

Refinement procedure

Resolution	30.000 * - 2.400
R-factor	0.211
Rwork	0.209
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o9g
RMSD bond length	0.010
RMSD bond angle	1.190 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.057 *	0.210 *
Total number of observations	19577 *
Number of reflections	8093 *	921 *
<I/σ(I)>	38.7	3.2
Completeness [%]	91.0 *	91 *
Redundancy	2.3	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.6	20 *	MES 6.6,PEG 20K 11%, pH 6.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	reservoir	MES	100 (mM)	pH6.6-6.9
3	1	reservoir	PEG20000	6-8 (%(w/v))