1O96

Structure of electron transferring flavoprotein for Methylophilus methylotrophus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-15
Spacegroup name	P 21 21 21
Unit cell lengths	117.516, 126.877, 221.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 3.100
R-factor	0.215
Rwork	0.212
R-free	0.27900 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.034 *
RMSD bond angle	2.940 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.08)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.200
High resolution limit [Å]	3.100	3.100
Rmerge	0.091	0.301
Total number of observations	553173 *
Number of reflections	58411
<I/σ(I)>	9.5	2.1
Completeness [%]	98.6	98.5
Redundancy	3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		SATURATED SODIUM CITRATE, pH 9.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG20000	30 (%(w/v))
2	1	reservoir	sodium acetate	0.2 (M)
3	1	reservoir	MES	0.1 (M)	pH6.5