1O92

Methionine Adenosyltransferase complexed with ADP and a L-methionine analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1999-07-15
Detector	MARRESEARCH
Spacegroup name	P 41 2 2
Unit cell lengths	115.140, 115.140, 160.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 3.000 *
R-factor	0.246
Rwork	0.246
R-free	0.26000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qm4
RMSD bond length	0.009
RMSD bond angle	24.700 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	3.450
High resolution limit [Å]	3.000 *	3.000 *
Rmerge	0.123 *	0.482 *
Number of reflections	20749 *
<I/σ(I)>	5	2.2
Completeness [%]	96.6	98.3 *
Redundancy	8.4	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		18% PEG 10000, 10MM ATP, 10MM LCISAMB, 0.1M HEPES PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH8.
3	1	drop	dithiothreitol	10 (mM)
4	1	drop		50 (mM)
5	1	drop		10 (mM)
6	1	drop	EDTA	1 (mM)
7	1	reservoir	PEG10000	16 (%(w/v))
8	1	reservoir	HEPES	100 (mM)	pH7.5