1O92
Methionine Adenosyltransferase complexed with ADP and a L-methionine analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1999-07-15 |
Detector | MARRESEARCH |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 115.140, 115.140, 160.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 * - 3.000* |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.26000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qm4 |
RMSD bond length | 0.009 |
RMSD bond angle | 24.700 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 3.450 |
High resolution limit [Å] | 3.000 * | 3.000 * |
Rmerge | 0.123 * | 0.482 * |
Number of reflections | 20749 * | |
<I/σ(I)> | 5 | 2.2 |
Completeness [%] | 96.6 | 98.3 * |
Redundancy | 8.4 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 18% PEG 10000, 10MM ATP, 10MM LCISAMB, 0.1M HEPES PH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 50 (mM) | pH8. |
3 | 1 | drop | dithiothreitol | 10 (mM) | |
4 | 1 | drop | 50 (mM) | ||
5 | 1 | drop | 10 (mM) | ||
6 | 1 | drop | EDTA | 1 (mM) | |
7 | 1 | reservoir | PEG10000 | 16 (%(w/v)) | |
8 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |