1O7X
Citrate synthase from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF NONIUS |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.340, 97.860, 119.330 |
Unit cell angles | 90.00, 107.60, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 * |
RMSD bond angle | 0.032 * |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.820 |
High resolution limit [Å] | 2.700 | 2.710 |
Rmerge | 0.072 | 0.229 |
Total number of observations | 148169 * | |
Number of reflections | 46758 | |
<I/σ(I)> | 9.4 | 3.2 |
Completeness [%] | 88.6 | 91.2 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.2 | pH 7.20 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | citrate | 10 (mM) | and CoA |
3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.2 |
4 | 1 | reservoir | PEG8000 | 17 (%(v/v)) | |
5 | 1 | reservoir | 0.1 (M) |