1O7V
High resolution structure of Siglec-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-29 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 40.049, 40.049, 140.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o7s |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.061 | 0.312 |
| Total number of observations | 31409 * | |
| Number of reflections | 9206 * | |
| <I/σ(I)> | 11.7 | 2.4 |
| Completeness [%] | 94.8 | 96.3 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | 0.2 M SODIUM ACETATE TRIHYDRATE 0.1 M SODIUM CACODYLATE PH 6.5, 30 % PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | pH8.0 |
| 3 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | trisodium citrate | 0.1 (M) | pH5.6 |
| 5 | 1 | reservoir | PEG4000 | 30 (%) | |
| 6 | 1 | reservoir | sodium acetate trihydrate | 0.2 (M) | |
| 7 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 8 | 1 | reservoir | PEG8000 | 30 (%) |
