1O7S
High resolution structure of Siglec-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.078, 53.078, 93.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qfo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.560 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.065 | 0.562 |
| Total number of observations | 86594 * | |
| Number of reflections | 14144 | |
| <I/σ(I)> | 8.6 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * | 0.2 M SODIUM ACETATE TRIHYDRATE 0.1 M TRI-SODIUM CACODYLATE, PH 6.5, 30 % PEG 8,000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | pH8.0 |
| 3 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | trisodium citrate | 0.1 (M) | pH5.6 |
| 5 | 1 | reservoir | PEG4000 | 30 (%) | |
| 6 | 1 | reservoir | sodium acetate trihydrate | 0.2 (M) | |
| 7 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
| 8 | 1 | reservoir | PEG8000 | 30 (%) |
