1O7K

human p47 PX domain complex with sulphates

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-28
Detector	ADSC CCD
Wavelength(s)	1.021409, 1.021248
Spacegroup name	I 2 2 2
Unit cell lengths	74.233, 92.175, 144.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.900 * - 2.000
R-factor	0.223
Rwork	0.210
R-free	0.27000 *
Structure solution method	MAD
RMSD bond length	0.020 *
RMSD bond angle	1.700 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.050 *	0.368
Total number of observations	83922 *
Number of reflections	24307 *
<I/σ(I)>	20.92	1.7
Completeness [%]	93.6 *	74.1
Redundancy	5.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5	4 *	used hair seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.0 (M)
2	1	reservoir	Tris	0.1 (M)	pH8.5
3	1	reservoir	glycerol	12 (%)