1O7A
Human beta-Hexosaminidase B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 163.931, 163.931, 244.717 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.120 - 2.250 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23600 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 23.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.065 | 0.356 |
| Total number of observations | 1930354 * | |
| Number of reflections | 180325 | |
| <I/σ(I)> | 25.4 | 5.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.7 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | HANGING DROP VAPOUR DIFFUSION PROTEIN SOLUTION: 10 MG/ML HEXB IN 10MM SODIUM CITRATE, 100 MM NACL, PH6.0 RESERVOIR SOLUTION: 100 MM SODIUM CITRATE, PH 5.6, 16% (V/V) ETHYLENE GLYCOL, 10%(V/V) POLYETHYLENE GLYCOL 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 2 | 1 | reservoir | ethylene glycol | 16 (%(v/v)) | |
| 3 | 1 | reservoir | PEG8000 | 10 (%(v/v)) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | sodium citrate | 10 (mM) | pH6.0 |
| 6 | 1 | drop | 100 (mM) |
