1O75
Tp47, the 47-Kilodalton Lipoprotein of Treponema pallidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-01 |
| Detector | APS SBC2 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 128.931, 128.931, 151.161 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 * - 1.950 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.22800 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.450 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.200 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.058 | 0.702 |
| Total number of observations | 478972 * | |
| Number of reflections | 105601 * | |
| <I/σ(I)> | 21.4 | 1.9 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 32% PEG 4000, 100 MM SODIUM CITRATE PH 5.6, 200 MM AMMONIUM ACETATE,3% (W/V) DEXTRAN SULFATE 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | pH7.4 |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | reservoir | PEG4000 | 32 (%(w/v)) | |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 6 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 7 | 1 | reservoir | dextran sulfate 8000 | 3 (%(w/v)) | |
| 8 | 1 | reservoir | 0.100 (mM) | plus or minus |
