1O73
Tryparedoxin from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.600, 31.460, 56.930 |
| Unit cell angles | 90.00, 93.43, 90.00 |
Refinement procedure
| Resolution | 56.800 - 2.300 * |
| Rwork | 0.177 |
| R-free | 0.24700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qk8 |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.450 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 2.360 |
| High resolution limit [Å] | 2.300 * | 2.280 |
| Rmerge | 0.073 | 0.217 |
| Total number of observations | 37965 * | |
| Number of reflections | 4699 * | |
| <I/σ(I)> | 19.6 | 6.5 |
| Completeness [%] | 98.7 | 94.7 |
| Redundancy | 8.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 50 MM HEPES PH 7.5, 30% PEG4000, 100 MM SODIUM ACETATE PH 4.6, 200 MM AMMONIUM ACETATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | pH7.5 |
| 3 | 1 | reservoir | PEG4000 | 30 (%) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 5 | 1 | reservoir | ammonium acetate | 200 (mM) |
