1O6U

The Crystal Structure of Human Supernatant Protein Factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-15
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	59.794, 84.240, 87.111
Unit cell angles	116.26, 102.39, 99.87

Refinement procedure

Resolution	20.000 * - 2.050
R-factor	0.204
Rwork	0.204
R-free	0.22700 *
Structure solution method	OTHER
RMSD bond length	0.005
RMSD bond angle	22.500 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.050
High resolution limit [Å]	2.050	2.000
Rmerge	0.093	0.251
Total number of observations	715326 *
Number of reflections	184572
<I/σ(I)>	15.7	3.9
Completeness [%]	97.1	97.1
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.4 *	18 *	pH 8.30

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	20 (%(w/v))
2	1	reservoir	glycerol	5 (%(w/v))
3	1	reservoir	sodium citrate	200 (mM)	pH8.3
4	1	drop	EDTA	2 (mM)
5	1	drop	dithiothreitol	2 (mM)
6	1	drop		40 (mM)
7	1	drop	Tris-HCl	20 (mM)	pH7.4
8	1	drop	protein	20 (mg/ml)