1O6P

Importin Beta bound to a GLFG Nucleoporin peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	125.820, 67.030, 129.260
Unit cell angles	90.00, 98.99, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.238
Rwork	0.238
R-free	0.26900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qgr
RMSD bond length	0.013
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.400
High resolution limit [Å]	2.800	2.800 *
Rmerge	0.062	0.314 *
Total number of observations	257406 *
Number of reflections	48209
<I/σ(I)>	7.9
Completeness [%]	91.8	89.6 *
Redundancy	2.6	2.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6		100MM AMMONIUM ACETATE PH6.0, 1.2M AMMONIUM SULPHATE, pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	peptide	25 (mM)
3	1	reservoir	ammonium acetate	100 (mM)	pH6.0
4	1	reservoir	ammonium sulfate	1.2 (M)