1O64
Crystal structure of an ATP phosphoribosyltransferase
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.183, 50.398, 76.794 |
| Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
| Resolution | 32.440 - 2.100 |
| Rwork | 0.227 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.000 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA) |
| Refinement software | REFMAC (4.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.440 | 2.210 |
| High resolution limit [Å] | 2.100 * | 2.100 |
| Rmerge | 0.033 | 0.088 |
| Number of reflections | 24260 | |
| <I/σ(I)> | 41.4 | 23.8 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 293 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | protein | 21.6mg/ml | |
| 2 | 1 | 2 | Tris | 20mM | |
| 3 | 1 | 2 | NaCl | 150mM | |
| 4 | 1 | 2 | beta-mercaptoethanol | 1mM | |
| 5 | 1 | 2 | methionine | 10mM |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | methionine | 10 (mM) | |
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | drop | dithiothreitol | 5 (mM) | |
| 6 | 1 | drop | protein | 10 (mg/ml) |
