1O5U
Crystal structure of a duf861 family protein (tm1112) from thermotoga maritima at 1.83 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-03 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.627, 74.729, 42.210 |
| Unit cell angles | 90.00, 91.02, 90.00 |
Refinement procedure
| Resolution | 27.980 - 1.830 |
| R-factor | 0.17277 |
| Rwork | 0.170 |
| R-free | 0.22200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lkn |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.650 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (5.0)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.980 * | 1.850 |
| High resolution limit [Å] | 1.830 * | 1.830 * |
| Rmerge | 0.045 * | 0.307 * |
| Total number of observations | 53783 * | |
| Number of reflections | 21322 | |
| <I/σ(I)> | 18 | 3.1 |
| Completeness [%] | 98.2 * | 84.6 * |
| Redundancy | 2.6 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.9 * | 277 | 25.5% PEG-4000, 0.17M ammonium sulfate, 15% glycerol anhydrous , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH7.9 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | TCEP | 0.25 (mM) | |
| 4 | 1 | drop | protein | 19 (mg/ml) | |
| 5 | 1 | reservoir | PEG4000 | 25.5 (%) | |
| 6 | 1 | reservoir | glycerol | 15 (%) | |
| 7 | 1 | reservoir | ammonium sulfate | 0.17 (M) |
