1O5T
Crystal structure of the aminoacylation catalytic fragment of human tryptophanyl-tRNA synthetase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.916, 0.9500, 0.9790, 0.9787 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 82.240, 82.240, 263.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.510 - 2.500 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.29600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.415 |
| Number of reflections | 18911 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 98.7 | 96.3 |
| Redundancy | 8.5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 277 | 16% PEG1500, 4% PEG3350, 0.2M tri-sodium citrate dihydrate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
