1O4W
CRYSTAL STRUCTURE OF a PIN (PILT N-TERMINUS) DOMAIN CONTAINING PROTEIN (AF0591) FROM ARCHAEOGLOBUS FULGIDUS AT 1.90 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98397, 0.97922, 0.91837, 0.97855 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.705, 63.705, 78.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.860 - 1.900 |
| R-factor | 0.19059 |
| Rwork | 0.188 |
| R-free | 0.23508 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.230 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (4.2)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.860 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 * | 0.638 * |
| Total number of observations | 131380 * | |
| Number of reflections | 14887 * | |
| <I/σ(I)> | 25.3 | 2.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 6 | 277 | Santarsiero, B.D., (2002) J. Appl. Crystallogr., 35, 278. * |
