1O3T
PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES
Replaces: 1DBCExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 258 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-09-01 |
| Detector | FUJI |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 136.990, 152.800, 76.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.2301 |
| Rwork | 0.230 |
| R-free | 0.30300 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | VRIED |
| Data scaling software | VRIED |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 14452 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | PEG 3350, MES, NACL, MGCL2, CACL2, CAMP, NAN3, DITHIOTHREITOL, SPERMINE, N-OCTYL-B-D-GLUCOPYRANOSIDE, pH 5.5, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | N-OCTYL-B-D-GLUCOPYRANOSIDE | ||
| 10 | 1 | 1 | PEG 3350 | ||
| 11 | 1 | 2 | PEG 3350 | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | CAMP | ||
| 6 | 1 | 1 | CACL2 | ||
| 7 | 1 | 1 | NAN3 | ||
| 8 | 1 | 1 | DITHIOTHREITOL | ||
| 9 | 1 | 1 | SPERMINE |
