1O3S
PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES
Replaces: 1DB9Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 108 |
Detector technology | IMAGE PLATE |
Collection date | 1997-09-01 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 78.050, 78.050, 142.790 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.2601 |
Rwork | 0.260 |
R-free | 0.30800 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.450 * |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.094 | 0.410 |
Total number of observations | 108811 * | |
Number of reflections | 10352 * | |
<I/σ(I)> | 4.9 | |
Completeness [%] | 98.4 | 94.3 |
Redundancy | 4.8 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.2 * | 20 * | Chen, S., (2001) J.Mol.Biol., 314, 63. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | 1,4-DIOXANE | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | NACL | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 1 | CAMP | ||
6 | 1 | 1 | PEG 8000 | ||
7 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | CAP | 0.1-0.2 (mM) | |
2 | 1 | drop | protein | 0.12-0.24 (mM) | |
3 | 1 | drop | cAMP | 2 (mM) | |
4 | 1 | drop | MES | 100 (mM) | pH6.2 |
5 | 1 | drop | 200 (mM) | ||
6 | 1 | drop | 50 (mM) | ||
7 | 1 | drop | PEG8000 | 5-6 (%(w/v)) | |
8 | 1 | drop | dioxane | 18-19 (%(v/v)) |