1O2E
Structure of the triple mutant (K53,56,120M) + Anisic acid complex of phospholipase A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 46.610, 46.610, 102.680 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.800 - 2.600 |
| Rwork | 0.187 |
| R-free | 0.24100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.151 | 0.263 |
| Number of reflections | 3957 | |
| Completeness [%] | 92.0 | 94.9 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion method | 7.2 | 293 | 50 mM Tris Buffer, MPD,70% MPD reservoir,17-20Mg/ml prot,5 mM CaCl2, 1 microlitre of anisic acid, pH 7.2, vapor diffusion method, temperature 293.0K |
