1O22
Crystal structure of an orphan protein (TM0875) from Thermotoga maritima at 2.00 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-13 |
| Detector | ADSC QUANTUM |
| Wavelength(s) | 0.97780, 0.91840, 0.97920 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.410, 58.410, 102.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.160 * - 2.000 |
| R-factor | 0.186 |
| Rwork | 0.182 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.440 * |
| Data reduction software | XFIT |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.160 * | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 * | 0.375 * |
| Total number of observations | 148070 * | |
| Number of reflections | 12462 | |
| <I/σ(I)> | 28.1 | 3.2 |
| Completeness [%] | 99.0 | 90.9 |
| Redundancy | 11.8 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.9 * | 293 | 25.5 % PEG 4000; 0.085 M Tris pH 8.5; 0.17 M Na(Ac), 15 % Glycerol, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH7.9 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | TCEP | 0.25 (mM) | |
| 4 | 1 | drop | protein | 9.4 (mg/ml) | |
| 5 | 1 | reservoir | PEG4000 | 25.5 (%) | |
| 6 | 1 | reservoir | Tris | 0.085 (M) | pH8.5 |
| 7 | 1 | reservoir | sodium acetate | 0.17 (M) | |
| 8 | 1 | reservoir | glycerol | 15 (%) |
