1O20

Crystal structure of Gamma-glutamyl phosphate reductase (TM0293) from Thermotoga maritima at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-20
Detector	ADSC QUANTUM
Wavelength(s)	0.9793, 0.9566, 0.9794
Spacegroup name	C 2 2 2
Unit cell lengths	105.150, 111.330, 86.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 * - 2.000
R-factor	0.163
Rwork	0.160
R-free	0.20300
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.532
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.087 *	0.600 *
Total number of observations	295973 *
Number of reflections	34597
<I/σ(I)>	20.46	3.33
Completeness [%]	99.3 *	99.5
Redundancy	8.55	7.44

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.4	25 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	mPEG5000	12 (%)
2	1	reservoir	Tris	10 (mM)	pH8.4