1O1N
Deoxy hemoglobin (A-GLYGLYGLY-C:V1M,L29W; B,D:V1M)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.229, 83.134, 53.798 |
| Unit cell angles | 90.00, 98.96, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.189 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c7b |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.014 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 | 0.245 |
| Number of reflections | 48004 | |
| <I/σ(I)> | 11.6 | 3.87 |
| Completeness [%] | 94.2 | 89.1 |
| Redundancy | 3.28 | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.50 |
