1O0Q
Crystal structure of a cold adapted alkaline protease from Pseudomonas TAC II 18, co-crystallized with 1 mM EDTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | H 3 |
| Unit cell lengths | 179.900, 179.900, 37.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.23700 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.560 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.900 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.099 * | 0.241 * |
| Total number of observations | 81355 * | |
| Number of reflections | 22772 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | Villeret, V., (1997) Protein Sci., 6, 2462. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0 |
| 3 | 1 | drop | protein | 15 (mg/ml) |
