1O0B
CRYSTAL STRUCTURE OF L-GLUTAMINE AND AMPCPP BOUND TO GLUTAMINE AMINOACYL TRNA SYNTHETASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-03-17 |
| Detector | UCSD MARK II |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 236.450, 92.890, 114.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.700 |
| R-factor | 0.2161 |
| Rwork | 0.216 |
| R-free | 0.27400 * |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.580 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (98.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.460 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.044 | 0.356 |
| Total number of observations | 139896 * | |
| Number of reflections | 48063 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 77.3 | 51.6 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 298 | Ammonium Sulfate, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 10 | 1 | drop | protein | 5 (mg/ml) | |
| 11 | 1 | drop | AMPCPP | 2 (mM) | |
| 2 | 1 | reservoir | PIPES | 20 (mM) | pH7.0 |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | beta-mercaptoethanol | 20 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 2.25 (M) | |
| 6 | 1 | drop | PIPES | 20 (mM) | pH7.0 |
| 7 | 1 | drop | 10 (mM) | ||
| 8 | 1 | drop | beta-mercaptoethanol | 20 (mM) | |
| 9 | 1 | drop | glutamine | 60 (mM) |
