1NZN

Cytosolic domain of the human mitchondrial fission protein Fis1 adopts a TPR fold

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	103
Detector technology	CCD
Collection date	2002-12-13
Detector	SBC-2
Wavelength(s)	0.96446
Spacegroup name	P 65 2 2
Unit cell lengths	48.985, 48.985, 169.199
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.230
R-free	0.25500 *
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.085
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.050
High resolution limit [Å]	2.500 *	2.000
Rmerge	0.044 *
Number of reflections	7912 *
Completeness [%]	99.7 *	99.8
Redundancy	7. *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5 *	298	Ammonium Sulphate, Sodium Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	32 (mg/ml)
2	1	drop	sodium acetate	25 (mM)
3	1	drop	dithiothreitol	2 (mM)	pH5.0
4	1	reservoir	sodium acetate	100 (mM)
5	1	reservoir	ammonium sulfate	2.0 (M)
6	1	reservoir	glycerol	5 (%)	pH4.6