1NZA
Divalent cation tolerance protein (Cut A1) from thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-01-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9 |
Spacegroup name | I 2 3 |
Unit cell lengths | 83.003, 83.003, 83.003 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.700 |
Rwork | 0.229 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kr4 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.073 | 0.479 |
Number of reflections | 230757 | |
<I/σ(I)> | 51.26 | 7.9 |
Completeness [%] | 99.8 | 100 |
Redundancy | 21.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.6 | 295 | NA ACETATE 1.65M, MES 0.1M , pH 6.6, MICROBATCH, temperature 295K |