1NZ3
K45E-K63E Variant of Horse Heart Myoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.000, 35.800, 125.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wla |
| RMSD bond length | 0.021 * |
| RMSD bond angle | 0.041 * |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.097 |
| Number of reflections | 14180 |
| Completeness [%] | 79.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 25 * | ammonium sulfate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 60 (%sat) | |
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | pH8.3 |
| 5 | 1 | reservoir | ammonium sulfate | 65 (%sat) | |
| 6 | 1 | reservoir | Tris-HCl | 20 (mM) | |
| 7 | 1 | reservoir | EDTA | 1 (mM) |
