1NZ0

RNASE P PROTEIN FROM THERMOTOGA MARITIMA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-11
Detector	ADSC
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	56.227, 64.143, 68.326
Unit cell angles	90.00, 102.02, 90.00

Refinement procedure

Resolution	50.000 * - 1.200
R-factor	0.1625
Rwork	0.163
R-free	0.21700
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	2.715 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.350
High resolution limit [Å]	1.200	1.200
Rmerge	0.059	0.565
Total number of observations	774818 *
Number of reflections	118678	16982 *
<I/σ(I)>	5.3	1.1
Completeness [%]	80.0	38.7
Redundancy	6.53	4.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2	23 *	PEG 1500, potassium sulfate, sodium acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		100 (mM)	pH4.8-5.2
2	1	reservoir	PEG1500	12-18 (%)
3	1	reservoir		200 (mM)
4	1	drop	protein	3 (mg/ml)