1NYW
The high resolution structures of RmlC from Streptoccus suis in complex with dTDP-D-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.586, 47.895, 72.417 |
| Unit cell angles | 90.00, 99.02, 90.00 |
Refinement procedure
| Resolution | 29.880 - 1.600 |
| R-factor | 0.17486 |
| Rwork | 0.173 |
| R-free | 0.21700 * |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.820 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.280 * | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.083 * | 0.292 * |
| Total number of observations | 383364 * | |
| Number of reflections | 43352 | |
| <I/σ(I)> | 5.2 | 1.8 |
| Completeness [%] | 95.7 | 79.8 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 298 | 0.1 M Tris, 4 mM NiCl2,40% PEG 2000, pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.4 |
| 3 | 1 | reservoir | 4 (mM) | ||
| 4 | 1 | reservoir | PEG2000 | 40 (%) | |
| 5 | 1 | drop | dTDP-D-glucose | 20 (mM) |
