1NY7
COWPEA MOSAIC VIRUS (CPMV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 298 |
| Detector technology | FILM |
| Detector | KODAK |
| Wavelength(s) | 1.5 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 317.000, 317.000, 317.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| Structure solution method | SIR and molecular replacement |
| RMSD bond length | 0.016 |
| RMSD bond angle | 27.400 * |
| Data reduction software | MGROSC |
| Data scaling software | MGROSC |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.180 |
| High resolution limit [Å] | 2.800 * | 3.000 |
| Rmerge | 0.109 * | |
| Total number of observations | 285379 * | |
| Number of reflections | 102333 * | |
| Completeness [%] | 85.5 | 71.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 298 | PEG 8000, potasium phosphate, ammonium sulfate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
| 2 | 1 | reservoir | PEG8000 | 2 (%(w/v)) | |
| 3 | 1 | reservoir | potassium phosphate | 0.05 (M) | pH7.0 |
| 4 | 1 | drop | protein | 35 (mg/ml) | |
| 5 | 1 | drop | potassium phosphate | 0.05 (M) | pH7.0 |
