1NY7

COWPEA MOSAIC VIRUS (CPMV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	298
Detector technology	FILM
Detector	KODAK
Wavelength(s)	1.5
Spacegroup name	I 2 3
Unit cell lengths	317.000, 317.000, 317.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.202
Rwork	0.202
Structure solution method	SIR and molecular replacement
RMSD bond length	0.016
RMSD bond angle	27.400 *
Data reduction software	MGROSC
Data scaling software	MGROSC
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.180
High resolution limit [Å]	2.800 *	3.000
Rmerge	0.109 *
Total number of observations	285379 *
Number of reflections	102333 *
Completeness [%]	85.5	71.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	298	PEG 8000, potasium phosphate, ammonium sulfate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.4 (M)
2	1	reservoir	PEG8000	2 (%(w/v))
3	1	reservoir	potassium phosphate	0.05 (M)	pH7.0
4	1	drop	protein	35 (mg/ml)
5	1	drop	potassium phosphate	0.05 (M)	pH7.0